Premium
Effect of ultrasound treatment on the extraction of insulin
Author(s) -
Zayas Joseph
Publication year - 1985
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260270818
Subject(s) - extraction (chemistry) , ultrasound , insulin , medicine , chromatography , chemistry , biochemical engineering , microbiology and biotechnology , biology , engineering , radiology
Insulin production requires a prolonged extraction process. An ultrasound method of insulin extraction was developed to increase efficiency. Application of ultrasound resulted in a significant increase in the extraction process as a result of pancreas tissue dispergation, destruction of the pancreas cells, intensive mixing, separation of particles, and increase in the interphase pancreas‐extractive medium. Optimal technological parameters of insulin extraction were established: ultrasound frequency, 19.5 kHz, specific intensity of ultrasound, 3.3 W/cm 2 ; time of ultrasound exposure, 5–10 min; and influence of cavitation process. Effectiveness of insulin extraction depends on the properties of the extractive medium. Optimal conditions were pH, 1.6–1.7, concentration of alcohol, 75%, temperature of extractive mixture, 13–17°C; ratio of ground pancreas to liquid extractive medium, 1 : 3.