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Shear stress effects on human embryonic kidney cells in Vitro
Author(s) -
Stathopoulos N. A.,
Hellums J. D.
Publication year - 1985
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260270713
Subject(s) - shear stress , laminar flow , shear (geology) , shear rate , biophysics , embryonic stem cell , chemistry , materials science , composite material , biology , biochemistry , mechanics , physics , viscosity , gene
Human embryonic kidney cells grown as an attached, confluent monolayer on a flat substrate were subjected to steady, uniform laminar flow of medium in a specially designed chamber in which flow patterns and shear stress are accurately defined and controlled. Experiments were performed for shear stress levels ranging from 0.2 to 6.0 N/m 2 with times of exposure to the shear stress ranging from 2 to 24 h. The influence of the shear field was slight at low shear stress (0.26 N/m 2 ). Higher stress levels (0.65 N/m 2 and higher) had significant effects on cell morphology, and on the post‐shear release of urokinase enzyme. Still higher stress levels (2.6 N/m 2 and higher) caused marked reduction in cell viability. These results may be of interest in addressing practical problems in developing commercial biosynthesis reactors.