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Enzyme adsorption in porous supports: Local thermodynamic equilibrium model
Author(s) -
Pedersen Henrik,
Furler Larry,
Venkatasubramanian K.,
Prenosil Jiri,
Stuker Ernst
Publication year - 1985
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260270706
Subject(s) - adsorption , porosity , porous medium , thermodynamics , enzyme , chemistry , distribution (mathematics) , chromatography , immobilized enzyme , ion exchange , chemical engineering , ion , materials science , organic chemistry , physics , mathematics , engineering , mathematical analysis
Enzyme adsorption from a finite bath (batch adsorption) onto porous spherical supports is investigated both experimentally and theoretically using β‐galactosidase and Duolite ion‐exchange resin as a model system. Efficient numerical techniques are presented that have been used in conjunction with a parameter estimation routine to evaluate adsorption isotherm constants. Results show that even for adsorption processes lasting almost 10 h, the majority of the enzyme is confined to the outer half of the support and, for high initial enzyme concentrations in the bath, this loading takes place as a slowly moving front. Information on the enzyme distribution has practical importance in the design of immobilized enzyme reactors that in previous works have almost always been analyzed assuming a uniform catalyst distribution.