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Kinetic characterization of the extracellular xylanases of Thermomonospora sp.
Author(s) -
Ristroph D. L.,
Humphrey A. E.
Publication year - 1985
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260270612
Subject(s) - xylose , xylobiose , xylan , thermophile , fermentation , chemistry , hydrolysis , enzyme , extracellular , xylanase , strain (injury) , chromatography , biochemistry , biology , anatomy
This study deals with characterizing the extracellular xylanases produced by a strain of the thermophilic bacterial genus Thermomonospora . Supernatant from centrifuged fermentation broth was used as a crude enzyme preparation. From pH 5.5 to pH 7.7 the temperature optimum based on a 10‐min assay of activity was 80°C. The crude enzyme had a half‐life of approximately 1 month when stored at 55°C at pH 6.5. The enzyme produced a mixture of xylose oligomers from xylan, with xylobiose occuring in greatest quantity on a molar basis. Only trace quantities of xylose were produced by this hydrolysis.