Kinetic characterization of the extracellular xylanases of  Thermomonospora  sp. | Zendy

Ristroph D. L. | Zendy; Humphrey A. E. | Zendy

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Kinetic characterization of the extracellular xylanases of Thermomonospora sp.

Author(s) -

Ristroph D. L.,

Humphrey A. E.

Publication year - 1985

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.260270612

Subject(s) - xylose , xylobiose , xylan , thermophile , fermentation , chemistry , hydrolysis , enzyme , extracellular , xylanase , strain (injury) , chromatography , biochemistry , biology , anatomy

This study deals with characterizing the extracellular xylanases produced by a strain of the thermophilic bacterial genus Thermomonospora . Supernatant from centrifuged fermentation broth was used as a crude enzyme preparation. From pH 5.5 to pH 7.7 the temperature optimum based on a 10‐min assay of activity was 80°C. The crude enzyme had a half‐life of approximately 1 month when stored at 55°C at pH 6.5. The enzyme produced a mixture of xylose oligomers from xylan, with xylobiose occuring in greatest quantity on a molar basis. Only trace quantities of xylose were produced by this hydrolysis.

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