Premium
Covalent coupling of microorganisms to a cellulosic support
Author(s) -
Okita W. Blair,
Bonham D. Bivings,
Gainer John L.
Publication year - 1985
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260270513
Subject(s) - cyanuric chloride , covalent bond , microorganism , cellulose , chemistry , cellulosic ethanol , bacteria , yeast , chemical engineering , coupling (piping) , materials science , organic chemistry , biochemistry , biology , composite material , genetics , engineering
Methods for the covalent coupling of microorganisms to a solid support were investigated. Both bacteria and yeast were attached to cellulose particles using cyanuric chloride as the coupling agent, although different experimental procedures were needed for the two types of microbes. This general technique for whole‐cell immobilization offers an advantage over entrapment methods in that the cells are attached to the outer surface of the solid, thus eliminating the resistance of a gel to the transfer of nutrients and products. There are also indications that such immobilized cells show high productivities.