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Application of scale‐down experiments in the study of kinetics of oxytetracycline biosynthesis
Author(s) -
Bošsnjak Marijan,
Stroj Alešs,
Caurčcica Miljenko,
Adamovica Vlasta,
Glunčcica Zdravka,
Bravar Danica,
Johanides Vera
Publication year - 1985
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260270403
Subject(s) - oxytetracycline , aeration , fermentation , biosynthesis , autolysis (biology) , yield (engineering) , chemistry , food science , biomass (ecology) , biochemistry , biology , antibiotics , materials science , organic chemistry , enzyme , agronomy , metallurgy
Scale‐down experiments in antibiotic biosynthesis were performed by transferring the corresponding amounts of fermentation broth from industrial to laboratory and pilot‐plant fermentors where the cultivation process was continued at different cultivation conditions. A previously proposed mathematical model was used to explain the experimental results. The effects of temperature, agitation‐aeration intensity, and medium addition during the process were investigated. Computer simulation data were fitted to the experimental data, and good agreement was found. As a consequence of increasing temperature up to 37°C, increases in the specific growth and autolysis rates as well as the specific rates of antibiotic synthesis and carbohydrate utilization were in evidence. Temperature increases of up to 40°C caused a lower oxytetracycline yield. The effect of increased oxygen transfer rate on oxytetracycline biosynthesis was more pronounced at higher temperatures than at lower cultivation temperatures. Culture differentiation (strain segregation) was also studied; it was found that the increased cultivation temperature could be favorable for the growth of biomass active in oxytetracycline biosynthesis. Results of experiments at the pilot‐plant scale showed that fed batch and repeated fed batch cultures could be successfully applied and the period of intensive antibiotic synthesis could be prolonged significantly.