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Simultaneous saccharification and fermentation of cellulose: Effect of ethanol on enzymatic saccharification of cellulose
Author(s) -
Ooshima Hiroshi,
Ishitani Yohsuke,
Harano Yoshio
Publication year - 1985
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260270402
Subject(s) - cellulase , hydrolysis , cellulose , chemistry , ethanol , fermentation , ethanol fuel , enzymatic hydrolysis , ethanol fermentation , biochemistry , food science , chromatography
It was confirmed that simultaneous saccharification and fermentation are effective for accelerating enzymatic saccharification of cellulose. In this work, the effects of ethanol on the saccharification of tissue paper by Trichoderma cellulase (Meicelase CEPB) have been investigated. The following results were obtained. (1) Saccharification was inhibited by at least 0.2 M ethanol. (2) Less than 4 M ethanol did not affect the enzymatic activities of β‐glucosidase and endoglucanase ( C x ) at all. The thermal stability of endoglucanase was not also varied by ethanol. (3) It is suggested that ethanol depresses the adsorption of exoglucanase on cellulose. (4) The rate expression of saccharification of cellulose in the presense of ethanol is proposed. (5) The inhibititory effect of ethanol was found to become more significant in the later stages of the reaction than just the initial stage.