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Nutrient‐limited continuous culture in the phauxostat
Author(s) -
Rice Craig W.,
Hempfling Walter P.
Publication year - 1985
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260270214
Subject(s) - saturation (graph theory) , phosphate , substrate (aquarium) , limiting , steady state (chemistry) , sulfate , chemistry , growth rate , escherichia coli , nutrient , population , biology , biochemistry , ecology , mathematics , organic chemistry , demography , combinatorics , sociology , gene , engineering , mechanical engineering , geometry
Stable steady‐state growth of Escherichia coli B limited by succinate, phosphate, or sulfate ion over the range of specific growth rates of 0.025–0.51 h −1 was achieved using pH‐controlled auxostasis in the phauxostat. The concentration of the growth‐limiting substrate in the growth vessel could be varied at will in the region of the Monod half‐maximal saturation constants by adjusting the concentration of that substrate in the reservior (at constant buffering capacity) or by varying the population density of the culture through changing the buffering capacity of the medium in the reservoir.