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Measurements of lactate concentration using lactate oxidase and an electrochemical oxygen sensor
Author(s) -
Makovos E. B.,
Liu C. C.
Publication year - 1985
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260270210
Subject(s) - oxygen , chemistry , electrochemistry , clark electrode , limiting oxygen concentration , chromatography , electrode , organic chemistry , electrolyte
Lactate oxidase was used in combination with an electrochemical dissolved oxygen sensor to measure L ‐lactate concentration in the physiological saline solution. The rate of oxygen consumption was found to have an excellent linear relationship with the lactate concentration on a log‐log scale in the lactate concentration range of 0.3–25 m M . This detection does not require additional reagents and can be developed into a simple method of L ‐lactate determination. The effects of the temperature and pH of the solution on the reaction rate were investigated and discussed.