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Kinetic characteristics of biochemical cyclic reaction systems: Amplification of substrate cycle system
Author(s) -
Okamoto Masahiro,
Hayashi Katsuya
Publication year - 1985
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260270205
Subject(s) - substrate (aquarium) , saturation (graph theory) , reaction rate constant , thermodynamics , chemistry , degree (music) , rate equation , limiting , reaction rate , kinetics , constant (computer programming) , kinetic energy , equilibrium constant , mathematics , physics , biochemistry , biology , catalysis , combinatorics , classical mechanics , computer science , ecology , acoustics , engineering , programming language , mechanical engineering
The amplification of a substrate cycle system with reversible closed reaction of two substrates was represented by mathematical equations. The results are summarized as follows: the amplification was affected especially by the affinity of enzyme and substrate, by the rate constant in rate‐limiting reaction step, and by the saturation degree of enzyme by substrate. These amplifications were not simply determined by the values of K m and V max , because each rate parameter in the system can affect the degree of amplification independently. The conclusion is that the “apparent” equilibrium constant of this system cannot be uniquely estimated from only data of K m and V max even if the reaction occurs in a closed system.