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Activity of Pseudomonas aeruginosa in biofilms: Steady state
Author(s) -
Bakke R.,
Trulear M. G.,
Robinson J. A.,
Characklis W. G.
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260261204
Subject(s) - biofilm , pseudomonas aeruginosa , steady state (chemistry) , substrate (aquarium) , dilution , chemistry , extracellular polymeric substance , microbiology and biotechnology , extracellular , biophysics , bacteria , biology , thermodynamics , biochemistry , ecology , physics , genetics
Aerobic glucose metabolism by Pseudomonas aeruginosa in steady‐state biofilms at various substrate loading rates and reactor dilution rates was investigated. Variables monitored were substrate (glucose), biofilm cellular density, biofilm extracellular polymeric substance (EPS) density, and suspended cellular and EPS concentrations. A mathematical model developed to describe the system was compared to experimental data. Intrinsic yield and rate coefficients included in the model were obtained from suspended continuous culture studies of glucose metabolism by P. aeruginosa . Experimental data compared well with the mathematical model, suggesting that P. aeruginosa does not behave differently in steady‐state biofilm cultures, where diffusional resistance is negligible, than in suspended cultures. This implies that kinetic and stoichiometric coefficients for P. aeruginosa derived in suspended continuous culture can be used to describe steady‐state biofilm processes.