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Cellular reporoduction and extracellular polymer formation by Pseudomonas aeruginosa in continuous culture
Author(s) -
Robinson Joseph A.,
Trulear Michael G.,
Characklis William G.
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260261203
Subject(s) - exopolymer , polymer , extracellular , chemistry , kinetics , pseudomonas aeruginosa , extracellular polymeric substance , growth rate , yield (engineering) , dilution , bacteria , biophysics , biofilm , biochemistry , chromatography , biology , organic chemistry , materials science , genetics , physics , geometry , mathematics , quantum mechanics , metallurgy , thermodynamics
The kinetics of cellular reproduction and the rate and extent of synthesis of extracellular polymeric substances (EPS) were investigated for P. aeruginosa growing in glucose‐limited chemostats. μ max and K s estimates of 0.4 h −1 and 2 mg glucose C/L, respectively, at 25°C were obtained for this bacterium. The extent of EPS formation was inversely related to the growth rate of P. aeruginosa . The rate of EPS formation had both growth‐ and non‐growth‐associated components. The growth‐associated polymer formation rate coefficient ( k ) was 0.3 mg polymer C/mg cellular C and the non‐growth‐associated polymer formation rate coefficient ( k ′) was 0.04 mg polymer C/mg cellular C/h. The values for k and k ′ must be regarded as provisional since the product formation data were quite variable at low dilution rates. Estimates of the cellular ( Y x/s ) and polymer ( Y p/s ) yield coefficients were 0.3 mg cellular C/mg glucose C and 0.6 mg polymer C/mg glucose C, respectively. Most of the non‐growth‐associated consumption of glucose detected was due to exopolymer formation.