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Enzymatic hydrolysis of cellulosic materials: A kinetic study
Author(s) -
Beltrame Pier Luigi,
Carniti Paolo,
Focher Bonaventura,
Marzetti Annamaria,
Sarto Virginio
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260261013
Subject(s) - cellulosic ethanol , hydrolysis , enzymatic hydrolysis , chemistry , enzyme , cellulose , biochemistry , organic chemistry
A kinetic study of the enzymatic hydrolysis of two celluloses with different structural features was performed at various temperatures (26–50°C). The enzymatic system consisted of three types of enzymes: E 1 —β‐1,4‐glucan glucanohydrolase; E 2 —β‐1,4‐glucan cellobiohydrolase; and E 3 —β‐glucosidase. A mathematical model for the mechanism of the hydrolysis of cellulosic materials catalyzed by a multienzymatic system was checked and a good rationalization of the experimental results was achieved. Uncompetitive and competitive glucose inhibition on E 1 and E 2 , respectively, appeared to occur for both substrates. Inhibition by cellobiose was checked at 34°C on one substrate. The V max , K m , and glucose inhibition constants were optimized and their dependence on temperature determined.