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Pretreatment of indian cane molasses for increased production of citric acid
Author(s) -
Kundu Subir,
Panda Tapobrata,
Majumdar S. K.,
Guha Barun,
Bandyopadhyay K. K.
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260915
Subject(s) - citric acid , chemistry , aspergillus niger , fermentation , hydrochloric acid , fractionation , cane , food science , organic acid , biochemistry , sugar , phosphate , yeast extract , yield (engineering) , chromatography , inorganic chemistry , materials science , metallurgy
Surface culture citric acid fermentation was carried out by Aspergillus niger T55, a strain isolated from its natural source, using cane molasses, either untreated or treated by various methods. Citric acid biosynthesis is seriously impaired by both organic and inorganic inhibitors. A combined treatment of molasses with tricalcium phosphate, hydrochloric acid, and Sephadex fractionation minimizes the level of inorganic and organic inhibitors in molasses and increases the production of citric acid (65% weight yield based on total reducing sugar). The optimum level of individual metal ions for citric acid production depends on the concentration of other metals in the medium.