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Production of ethyl acetate from dilute ethanol solutions by Candida utilis
Author(s) -
Armstrong David W.,
Martin Stanley M.,
Yamazaki Hiroshi
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260905
Subject(s) - ethyl acetate , ethanol , chemistry , solvent , ethanol fuel , chromatography , aeration , organic chemistry , nuclear chemistry
The conversion of ethanol to ethyl acetate has an advantage as a method of ethanol recovery since ethyl acetate is amenable to simple solvent extraction. The potential of Candida utilis in this conversion was studied. The kinetics of accumulation of ethanol and ethyl acetate in glucose‐grown C. utilis showed that ester formation resulted from ethanol utilization under appropriate aeration and was inhibited by Fe 3+ supplementation. Candida utilis converted ethanol to ethyl acetate optimally at pH 5.0–7.0. The five‐hour rate of ester production increased as the ethanol concentration increased to 10 g/L, and rapidly declined to zero at concentrations exceeding 35 g/L. Thus, C. utilis has potential to recover dilute ethanol in the form of ethyl acetate.