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Effect of dilution on carboxymethylcellulase and xylanase assays
Author(s) -
Cauchon Nicole,
Leduy Anh
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260825
Subject(s) - xylanase , trichoderma viride , cellulase , dilution , aspergillus niger , enzyme , chromatography , enzyme assay , chemistry , food science , microbiology and biotechnology , biochemistry , biology , physics , thermodynamics
It has been demonstrated that the dilution of samples prior to the carboxymethylcellulase and xylanase assays causes serious discrepancies in the numerical values obtained for the enzyme activities. Even when the sample is assayed with the identical procedure, one could obtain different numerical values of the enzyme activity U depending on how much this sample has been diluted before the enzyme assay. Two crude commercial cellulase samples of Aspergillus niger and Trichoderma viride as well as the culture filtrate of our newly isolated acidophilic fungus have been used for the demonstration. An empirical method for reporting the cellulolytic activity by taking into account this dilution effect is proposed.