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Continuous growth kinetics of Candida utilis in pineapple cannery effluent
Author(s) -
Prior B. A.
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260718
Subject(s) - dilution , chemostat , chemistry , sucrose , saturation (graph theory) , sugar , effluent , pomace , chromatography , food science , botany , biology , environmental engineering , physics , genetics , mathematics , combinatorics , engineering , bacteria , thermodynamics
Candida utilis was grown on a pineapple cannery effluent as the sole carbon and energy source in a chemostat at dilution rates between 0.10 and 0.62 h −1 to determine the growth kinetics. The principal sugars in the effluent were sucrose, glucose, and fructose. The cell yield coefficient on carbohydrate varied with dilution rate and a maximum value of 0.63 was observed at a dilution rate of 0.33 h −1 . The steady‐state concentrations of carbohydrate, reducing sugar, and chemical oxygen demand (COD) appeared to follow Monod saturation kinetics with increasing dilution rate, although none of the measured parameters represented a pure substrate. The maximum specific growth rate and reducing sugar saturation constant were 0.64 h −1 and 0.060 g/L, respectively. A maximum cell mass productivity of 2.3 g/L h was observed at a dilution rate of 0.51 h −1 . At this dilution rate, only 68% of the COD was removed. A 95% COD removal was attained at a dilution rate of 0.10 h −1 . Optimal yeast productivity and COD reduction occurred at a dilution rate of 0.33 h −1 .