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Use of catalase from Escherichia coli in model experiments for oxygen supply of microorganisms with hydrogen peroxide
Author(s) -
Nies D.,
Schlegel H. G.
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260716
Subject(s) - catalase , hydrogen peroxide , escherichia coli , microorganism , chemistry , oxygen , microbiology and biotechnology , peroxide , bacteria , biochemistry , biology , enzyme , organic chemistry , genetics , gene
A catalase has been purified from aerobically grown Escherichia coli K12. The enzyme exhibits unorthodox properties compared with catalyse from bovine liver and seems to be identical to hydroperoxidase II from E. coli . A mathematical model is presented which makes it possible to calculate the steady‐state concentration of hydrogen peroxide in an open system. The model has been verified experimentally. It has been shown that the catalase from E. coli is better suited than the bovine liver enzyme for oxygen supply to cell suspensions using hydrogen peroxide.