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Mechanisms for the acoustic conditioning of protein precipitates to improve their separation by centrifugation
Author(s) -
Bell D. J.,
Dunnill P.
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260710
Subject(s) - dewatering , centrifugation , settling , conditioning , chromatography , isoelectric point , materials science , particle (ecology) , particle aggregation , particle size , aggregate (composite) , isoelectric focusing , chemistry , composite material , thermodynamics , biology , nanotechnology , mathematics , physics , biochemistry , engineering , ecology , nanoparticle , enzyme , statistics , geotechnical engineering
A theoretical description of a low‐frequency, dual‐source acoustic treatment of protein precipitates is presented. Two mechanisms for the potential improvement of the centrifugal separation of precipitates are proposed. They are acoustic aggregation, which causes an increase in particle size, and aggregate deformation, which results in an increase in aggregate density. Experiments with isoelectric soya protein precipitate indicated that the extent of ageing prior to acoustic conditioning is important. Support for both aggregation and dewatering was found and a maximum increases in the particle settling velocity of 20% was observed.