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DNA immobilized onto an acyl‐azide derivative of collagen membranes for use as immunoadsorbent
Author(s) -
El Habib Raphaelle,
Coulet Pierre R.,
Sanhadji Kamel,
Gautheron Danielle C.,
Laville Maurice,
Traeger J.
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260705
Subject(s) - dna , chemistry , covalent bond , membrane , azide , in vitro , derivative (finance) , polymerization , covalent binding , chromatography , biochemistry , organic chemistry , polymer , financial economics , economics
Single‐ and double‐stranded DNA were immobilized on films of highly polymerized collagen by a covalent process. The coupling was efficient at an acidic pH with an optimum at pH 5, while preventing the collagen film from any damage. In addition, no leakage occurred, so it was possible to use this DNA‐coated collagen film as an immunoadsorbent. Therefore the findings reported here suggest that the acyl‐azide procedure is also suitable for DNA binding on a proteinic support. Promising results in specific clearance of DNA antibodies were obtained in vitro .