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Invertase covalent grafting onto corn stover
Author(s) -
Monsan P.,
Combes D.,
Alemzadeh I.
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260704
Subject(s) - invertase , chemistry , sodium cyanoborohydride , corn stover , reductive amination , hydrolysis , sucrose , immobilized enzyme , amination , chromatography , nuclear chemistry , organic chemistry , enzyme , catalysis
The covalent coupling of an invertase from baker's yeast onto an agricultural by‐product, corn grits, has been developed. The optimal conditions for each step of the chemical modification of the support have been determined: oxidation with sodium metaperiodate, amination with ethylenediamine, reduction with sodium cyanoborohydride, and activation with glutaraldehyde. Activities up to 7.2 × 10 4 μmol reducing sugars produced/min g support could thus be achieved. Invertase coupling onto corn grits yields a derivative with a 25 times higher activity than when coupling this enzyme onto porous silica. The operational stability of invertase immobilized onto corn stover was found to be very high, with a half‐life of up to 365 days at 40°C when using a 2 M sucrose solution as substrate. This immobilization method could be easily scaled up to the preparation of 10 kg of invertase derivative.