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Streptomyces flavogriseus cellulase: Evaluation under various hydrolysis conditions
Author(s) -
MacKenzie C. R.,
Bilous D.,
Johnson K. G.
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260605
Subject(s) - cellulase , cellobiose , cellulose , hydrolysis , chemistry , substrate (aquarium) , enzyme , streptomyces , beta glucosidase , chromatography , streptomycetaceae , actinomycetales , food science , nuclear chemistry , biochemistry , bacteria , biology , genetics , ecology
Streptomyces flavogriseus CMCase and Avicelase were very stable at 30°C but not at 40°C or higher. β‐Glucosidase was less stable at all temperatures tested. Stabilities were similar at pH values between 5.5 and 7, the optimal range for enzyme activity. Cellulose solubilizing activity was reduced by 40% at a cellobiose concentration of 150m M but glucose inhibited activity by only 10% at this concentration. β‐Glucosidase was inhibited by 40% at a glucose concentration of 10m M (ten times the substrate concentration). Relatively dilute S. flavogriseus cellulase extensively hydrolysed acid‐swollen cellulose at concentrations as high as 10%. More highly crystalline forms of cellulose were more resistant to attack.