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The hydroxylation of biphenyl by Aspergillus toxicarius : Conditions for a bench scale fermentation process
Author(s) -
Golbeck John H.,
Cox John C.
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260506
Subject(s) - industrial fermentation , fermentation , chemistry , aeration , hydroxylation , laboratory flask , biomass (ecology) , biphenyl , substrate (aquarium) , food science , pulp and paper industry , aspergillus , organic chemistry , biology , botany , enzyme , agronomy , ecology , engineering
Fungi of the Aspergillus sp. can hydroxyate biphenyl to 4,4′‐dihydroxybiphenyl, a chemical intermediate used in the plastics industry. The authors studied various batch culture conditions for the production of 4,4′‐dihydroxybiphenyl, by Aspergillus toxicarius , in 25‐mL shake flasks and 2‐L fermenter cultures. Conditions investigated included temperature, aeration, carbon and nitrogen sources, biomass content, and time of substrate addition. Under optimum conditions we observed a rate of 4,4′‐dihydroxybiphenyl production of 15–20 mg/day/g dry wt mycelia. Such a production rate is probably too low to support a commercial process and possible reasons for the low productivity are discussed.