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Computer model for glucose‐limited growth of a single cell of Escherichia coli B/r‐A
Author(s) -
Domach M. M.,
Leung S. K.,
Cahn R. E.,
Cocks G. G.,
Shuler M. L.
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260303
Subject(s) - cell division , biological system , dna synthesis , cell cycle , escherichia coli , mechanism (biology) , biology , growth model , cell growth , chromosome , computational biology , division (mathematics) , cell , model system , dna , biochemical engineering , biochemistry , microbiology and biotechnology , mathematics , gene , engineering , arithmetic , philosophy , mathematical economics , epistemology
A computer model is described which is capable of predicting changes in cell composition, cell size, cell shape, and the timing of chromosome synthesis in response to changes in external glucose limitation. The model is constructed primarily from information on unrestricted growth in glucose minimal medium. The ability of the model to make reasonable quantitative predictions under glucose‐limitation is a test of the plausibility of the basic biochemical mechanisms included in the model. Such a model should be of use in differentiating among competing hypotheses for biological mechanisms and in suggesting as yet unobserved phenomena. The last two points are illustrated with the testing of a mechanism for the control of the initiation of DNA synthesis and predictions on cellwidth variations during the division cycle.