Isolation of an inulinase derepressed mutant of  Pichia polymorpha  for the production of fructose | Zendy

Bajon A. M. | Zendy; Guiraud J. P. | Zendy; Galzy P. | Zendy

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Isolation of an inulinase derepressed mutant of Pichia polymorpha for the production of fructose

Author(s) -

Bajon A. M.,

Guiraud J. P.,

Galzy P.

Publication year - 1984

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.260260204

Subject(s) - inulinase , inulin , fructose , mutant , biochemistry , chemistry , hydrolysis , enzyme , biology , gene

Pichia polymorpha has inulinase activity and could be used for the production of fructose syrup from inulin. The application of immobilized P. polymorpha whole cells for the continuous hydrolysis of inulin is, however, limited since the biosynthesis of this enzyme system is repressed by the reaction products, dextrose and fructose. A derepressed mutant hyperproducer of inulinase was isolated after treatment with EMS followed by a selection step with deoxyglucose.

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