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Isolation of an inulinase derepressed mutant of Pichia polymorpha for the production of fructose
Author(s) -
Bajon A. M.,
Guiraud J. P.,
Galzy P.
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260204
Subject(s) - inulinase , inulin , fructose , mutant , biochemistry , chemistry , hydrolysis , enzyme , biology , gene
Abstract Pichia polymorpha has inulinase activity and could be used for the production of fructose syrup from inulin. The application of immobilized P. polymorpha whole cells for the continuous hydrolysis of inulin is, however, limited since the biosynthesis of this enzyme system is repressed by the reaction products, dextrose and fructose. A derepressed mutant hyperproducer of inulinase was isolated after treatment with EMS followed by a selection step with deoxyglucose.