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Kinetics of formation of maltose and isomaltose through condensation of glucose by glucoamylase
Author(s) -
Adachi Shuji,
Ueda Yasuo,
Hashimoto Kenji
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260203
Subject(s) - isomaltose , maltose , chemistry , hydrolysis , aspergillus niger , biochemistry , kinetics , condensation , enzyme , thermodynamics , physics , quantum mechanics
A kinetic model was devised for the hydrolysis and synthesis of maltose and isomaltose by two glucoamylases from Rhizopus niveus and Aspergillus niger , and the validity of the model was verified experimentally at 313 K and pH 5.0. For both enzymes, the formations of maltose and isomaltose from glucose were parallel reversible reactions, and glucosyl transfer between maltose and isomaltose was not observed. The enzymes catalyzed rapid hydrolysis and synthesis of maltose. Isomaltose was hydrolyzed and synthesized more slowly, but the level produced from glucose was much higher than that of maltose. These hydrolysis and condensation reactions were expressed well by the model.