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Environmental parameters influencing phenolics production by batch cultures of Nicotiana tabacum
Author(s) -
Sahai O. P.,
Shuler M. L.
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260202
Subject(s) - production (economics) , biology , chemistry , botany , food science , microbiology and biotechnology , biochemical engineering , engineering , economics , macroeconomics
Abstract The influence of temperature, illumination, hormonal levels (2,4‐D and kinetin), carbon to nitrogen ratios, antibiotics, and precursor feeding on phenolics production by Nicotiana tabacum (tobacco) was studied. This plant cell system was chosen as a model system to learn more about secondary product formation in plant cell tissue cultures. This is the first study to manipulate all of these environmental parameters with a single plant cell system. The most striking results were with 2,4‐D manipulation. The removal of 2,4‐D resulted in significant phenolics production during the stationary phase, while normal levels strongly suppressed phenolics production during the stationary phase. The addition of phenylalanine stimulated phenolics production per gram of cells but strongly inhibited growth.