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Effect of dissolved oxygen on nitrogen fixation by A. vinelandii . II. Ionically adsorbed cells
Author(s) -
DiLuccio Robert C.,
Kirwan Donald J.
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260116
Subject(s) - azotobacter vinelandii , nitrogenase , oxygen , nitrogen fixation , chemistry , nitrogen , adsorption , respiration , lysis , cellular respiration , biochemistry , biophysics , nuclear chemistry , biology , botany , organic chemistry , mitochondrion
Continuous culture studies of Azotobacter vinelandii cells immobilized by ionic adsorption to Cellex E anion exchange resin were conducted under oxygen‐limited conditions for comparison to free‐cell cultures. Immobilization had little effect upon the specific respiration and sucrose consumption rates as compared to free cells. However, maxima in specific nitrogen fixation rate and nitrogenase activity as a function of dissolved oxygen occurred at a C O 2value of approximately 0.005 m M as opposed to 0.02 m M for free cells. Further, in contrast to free‐cell culture, most of the fixed nitrogen appeared in the medium rather than within intact cells. There were strong indications that reproduction of bound cells often resulted in cell lysis accounting for the fixed nitrogen content in solution.