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Effect of dissolved oxygen on nitrogen fixation by A. vinelandii . I. Free cell cultures
Author(s) -
DiLuccio Robert C.,
Kirwan Donald J.
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260115
Subject(s) - azotobacter vinelandii , nitrogenase , nitrogen fixation , oxygen , nitrogen , respiration , azotobacter , chemistry , cellular respiration , mesophile , ammonia , environmental chemistry , botany , bacteria , biochemistry , biology , genetics , organic chemistry
As part of an investigation into the use of biological nitrogen fixation for fertilizer ammonia production, continuous culture studies of respiration and nitrogen fixation in the aerobic bacteria Azotobacter vinelandii under oxygen‐limited conditions were conducted. Respiration and growth rates followed Monod forms with respect to dissolved oxygen concentration. However, specific nitrogen fixation rate and nitrogenase activity exhibited maximum values at dissolved oxygen concentrations of ca. 0.02 m M (10% of air saturation). These results suggest careful control of oxygen in the environment is necessary to optimize fixed nitrogen production by this organism.