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Cellulase production from immobilized growing cell composites prepared by radiation polymerization
Author(s) -
Kumakura Minoru,
Kaetsu Isao,
Nisizawa Kazutosi
Publication year - 1984
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260260105
Subject(s) - cellulase , polymerization , trichoderma reesei , chemistry , materials science , cellulose , polymer , hydrolysis , composite material , monomer , chemical engineering , organic chemistry , engineering
The cellulase production from immobilized Trichoderma reesei composites prepared by radiation polymerization at low temperature was studied. The production of cellulase from the cells irradiated by radiation was slightly retarded at the initial stage of the culture, but was immediately recovered. The production of cellulase resulting from the growth of the immobilized cells proceeds efficiently in the composite having a porous polymer matrix, in which the productivity of cellulase varied with the hydrophilicity, the shape of the composite, monomer, and cell concentration. It was found that cellulase produced by immobilized growing cell composites effectively hydrolyzed cellulosic wastes such as newspaper and chaff which are pretreated by irradiation and crushing.