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Explosive pretreatment of lignocellulosic residues with high‐pressure carbon dioxide for the production of fermentation substrates
Author(s) -
Puri V. P.,
Mamers H.
Publication year - 1983
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260251226
Subject(s) - bagasse , hemicellulose , woodchips , chemistry , pulp and paper industry , lignin , cellulose , straw , raw material , steam explosion , enzymatic hydrolysis , fermentation , hydrolysis , cellulase , carbon dioxide , food science , organic chemistry , inorganic chemistry , engineering
A new pretreatment technique has been developed in which the lignocellulosic material is subjected to the action of steam and high‐pressure carbon dioxide before being explosively discharged through a defibrating nozzle of novel design. Operating at 200°C and gas pressures in the range of 3.45–13.8 MPa, exploded products with maximum in vitro cellulase digestibilities of 81, 78, and 75% were obtained from wheat straw, bagasse, and Eucalyptus regnans woodchips, respectively. The treatment times required to obtain substrates of maximum digestibility were 5 min for wheat straw and bagasse and 15 min for E. regnans . Analysis of the exploded products indicated that the pretreatment had substantially removed and solubilized the hemicellulose fraction of the feed materials, giving an autohydrolysis liquor rich in xylose and a fibrous residue primarily composed of α‐cellulose and lignin. The fibrous residue was readily amenable to cellulase hydrolysis, with saccharification being completed within 48 h. The theoretical energy demand for gas compression in the pretreatment process has been calculated at 11 k Wh/ton raw material when the digester is operated at 3.45 MPa and a packing density of 250 kg raw material/m 3 digester volume.

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