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Isoelectric precipitation of soy protein. II. Kinetics of protein aggregate growth and breakage
Author(s) -
Petenate Angela M.,
Glatz Charles E.
Publication year - 1983
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260251220
Subject(s) - breakage , chemistry , coulter counter , turbulence , particle (ecology) , ionic strength , aggregate (composite) , population , chromatography , residence time (fluid dynamics) , growth rate , thermodynamics , materials science , physics , composite material , mathematics , microbiology and biotechnology , engineering , biology , geology , geometry , oceanography , demography , geotechnical engineering , sociology , aqueous solution
Abstract Protein aggregate growth and breakage in agitated suspensions are modeled. The model includes growth of particles by a turbulent collision mechanism and breakage by a hydrodynamic shear mechanism. In the model, breakage results in the splitting of the particles into several small fragments. The model parameters are a growth rate constant and a breakage rate constant. Aggregate size distributions were measured with a Coulter counter and the data interpreted using a population balance that governs the steady‐state particle size distribution in a continuous stirred tank reactor. Effects of changes in the operating variables pH, concentration, mean residence time, ionic strength, and mixing power input on the model kinetic parameters are investigated.