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Development of a culture medium for large‐scale production of cellulolytic enzymes by Trichoderma reesei
Author(s) -
Warzywoda M.,
Ferre V.,
Pourquie J.
Publication year - 1983
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260251216
Subject(s) - trichoderma reesei , lactose , enzyme , cellulase , cellulose , chemistry , food science , enzyme assay , inducer , cellulosic ethanol , biochemistry , gene
Culture filtrates of CL‐847 strain of Trichoderma reesei grown on different carbon sources have been compared. The highest enzyme production is obtained with Whatman C 41 cellulose: 17.9 mg/mL of soluble proteins and 13.7 units of filter paper (FP) activity. Wood pulps gave lower production values and more viscous culture media. About one‐third of maximal enzyme production is obtained on lactose as the sole carbon source. Addition of 0.5% cellulosic inducer to 6% lactose media enhances enzyme production up to the following levels: 14.1 mg/mL of soluble proteins and 8.4 units of FP activity.