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Reaction characteristics of an immobilized yeast producing ethanol
Author(s) -
Furusaki Shintaro,
Seki Minoru,
Fukumura Kenichi
Publication year - 1983
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260251209
Subject(s) - yeast , michaelis–menten kinetics , saccharomyces cerevisiae , ethanol , polyacrylamide , chemistry , chromatography , polyacrylamide gel electrophoresis , biochemistry , enzyme , polymer chemistry , enzyme assay
The reaction behavior of Saccharomyces formaosensis imobilized by polyacrylamide gel is presented. Two types of the immobilized yeast are studied, i. e. the immobilized resting yeast and the immobilized growing yeast. For both of the yeast, reaction retes are expressed by the Michaelis‐Menten type equation with a linear ethanol inhibition factor. The Michaelis constants aere close each other, but considerably larger that of native S. cerevisiae. Distribution of the growing yeast cell inside the carrier gel is presented. It is found that the cell density is somewhat higher near the surface of the carrier.

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