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Effect of cellobiose, glucose, ethanol, and metal ions on the cellulase enzyme complex of Thermomonospora fusca
Author(s) -
Ferchak John D.,
Pye E. Kendall
Publication year - 1983
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260251205
Subject(s) - cellobiose , cellulase , cellulose , chemistry , cellulosic ethanol , ethanol , hydrolysis , metal ions in aqueous solution , enzyme , metal , biochemistry , nuclear chemistry , chromatography , food science , organic chemistry
The saccharification of cellulosic substrates by cellulase from Thermomonospora fusca , strain YX, was influenced by the presence of various substances. Cellobiose was strongly inhibitory, reducing the activity against swollen cellulose to 25% at 5% concentration. Glucose had much less effect, reducing activity to 40% at 20% concentration. Ethanol was found to be only slightly inhibitory to the cellulase, reducing activity by about 15% at 6% concentration, but much more inhibitory to the cell‐bound β‐glucosidase activity. Of the numerous metal ions examined, Ca 2+ and Co 2+ at 0.1m M ‐1.0m M concentration were found to be slightly activating under assay conditions, while 1.0m M Pb 2+ and Hg 2+ were the most inhibitory ions. The implications of these results for the design of commercial processes for ethanol production from cellulose are discussed.