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Enzyme electrode for specific determination of L ‐lysine
Author(s) -
Romette J. L.,
Yang J. S.,
Kusakabe H.,
Thomas D.
Publication year - 1983
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260251106
Subject(s) - lysine , trichoderma viride , enzyme , chemistry , electrode , clark electrode , gelatin , immobilized enzyme , enzyme assay , fermentation , membrane , chromatography , biochemistry , amino acid , food science , electrolyte
L ‐Lysine α‐oxidase from Trichoderma viride Y244‐2 is immobilized in a gelatin support and fixed on a pO 2 sensor. The enzyme electrode obtained is used in a continuous flow system in order to measure the concentration of L ‐lysine in a fermentor. The sample oxygen‐content dependance of the signal is minimized because of the enzyme support properties. The enzyme electrode response is set for lysine concentration from 0.2m M to 4m M . The specificity of lysine is tested with other amino acids. The enzyme membrane for lysine electrode can be used 3000 times or stored six months with good stability.