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Kinetic study on enzymatic hydrolysis of cellulose by cellulose from Trichoderma viride
Author(s) -
Ohmine Kazuo,
Ooshima Hiroshi,
Harano Yoshio
Publication year - 1983
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260250813
Subject(s) - trichoderma viride , cellulose , cellulase , hydrolysis , enzymatic hydrolysis , chemistry , cellulosic ethanol , crystallinity , product inhibition , chromatography , organic chemistry , enzyme , food science , non competitive inhibition , crystallography
Pure cellulose (Avicel) was hydrolyzed batchwise at 50°C and pH 4.8 by cellulase from Trichoderma viride (Meicelase CEP). Then the effects of the crystallinity of cellulose as well as the thermal deactivation and product (cellubiose and glucose) inhibition to cellulose on the hydrolysis rate were quantitatively investigated. While these factor had evidently retarded the enzymatic hydrolysis of cellulose to a significant extent, the hydrolysis rates observed could not be explained. For practical purposes, an empirical, simple rate expression was developed which included only one parameter: a overall rate retardation constant. This empirical rate expression held for the hydrolysis of at least two kind of cellulosic materials: Avicel and tissue paper.