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A Process for large‐scale isolation of β‐galactosidase from E. coli in an aqueous two‐phase system
Author(s) -
Veide Andres,
Smeds AnnaLisa,
Enfors SvenOlof
Publication year - 1983
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260250709
Subject(s) - nucleic acid , potassium phosphate , peg ratio , chromatography , chemistry , isolation (microbiology) , aqueous solution , potassium , phase (matter) , suspension (topology) , salt (chemistry) , phosphate , biochemistry , microbiology and biotechnology , biology , organic chemistry , mathematics , finance , homotopy , pure mathematics , economics
A method suitable for large‐scale isolation of β‐galactosidase from a suspension of disintegrated E. coli cells has been developed. In an aqueous two‐phase system consiting of PEG 6000 and potassium phosphate, all cell debris and the major part of the proteins and nucleic acids were partitioned to the denser salt phase. Seventy‐five percent of the β‐galactosidase was recovered in the lighter PEG phase, giving a purification ratio of about 12.