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Kinetics of multilayer immobilized enzyme‐filter reactors: Behavior of urease‐filter reactors in different buffers
Author(s) -
Goldstein Leon,
Levy Mordechai,
Shemer Lev
Publication year - 1983
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260250606
Subject(s) - urease , substrate (aquarium) , filter (signal processing) , kinetics , immobilized enzyme , chemistry , urea , product inhibition , filter paper , filter press , chromatography , enzyme , kinetic energy , chemical engineering , combinatorial chemistry , biochemistry , non competitive inhibition , computer science , biology , physics , engineering , ecology , quantum mechanics , computer vision
A sequential model which describes the kinetic behavior of multilayer immobilized‐enzyme filter reactors for systems obeying the Michaelis‐Menten scheme and various types of inhibition by product and by substrate is proposed. The model was varified experimentaly using a single enzyme‐filter‐substrate system—urease bound to nylon‐filter disks acting on urea—in different buffers to obtain the various inhibition patterns.