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A simple, sensitive, and accurate alcohol electrode
Author(s) -
Verduyn C.,
Van Dijken J. P.,
Scheffers W. A.
Publication year - 1983
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260250413
Subject(s) - electrode , chemistry , clark electrode , standard hydrogen electrode , alcohol , quinhydrone electrode , ethanol , alcohol oxidase , palladium hydrogen electrode , aqueous solution , reference electrode , analytical chemistry (journal) , inorganic chemistry , chromatography , organic chemistry , electrochemistry , biochemistry , pichia pastoris , electrolyte , gene , recombinant dna
The construction and performance of an enzyme electrode is described which specifically detects lower primary aliphatic alcohols in aqueous solutions. The electrode consists of a commercial Clark‐type oxygen electrode on which alcohol oxidase (E.C. 1.1.3.13) and catalase were immobilized. The decrease in electrode current is linearly proportional to ethanol concentrations between 1 and 25 ppm. The response of the electrode remains constant during 400 assays over a period of two weeks. The response time is between 1 and 25 min. Assembly of the electrode takes less than 1 h.