A simple, sensitive, and accurate alcohol electrode | Zendy

Verduyn C. | Zendy; Van Dijken J. P. | Zendy; Scheffers W. A. | Zendy

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A simple, sensitive, and accurate alcohol electrode

Author(s) -

Verduyn C.,

Van Dijken J. P.,

Scheffers W. A.

Publication year - 1983

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.260250413

Subject(s) - electrode , chemistry , clark electrode , standard hydrogen electrode , alcohol , quinhydrone electrode , ethanol , alcohol oxidase , palladium hydrogen electrode , aqueous solution , reference electrode , analytical chemistry (journal) , inorganic chemistry , chromatography , organic chemistry , electrochemistry , biochemistry , pichia pastoris , electrolyte , gene , recombinant dna

The construction and performance of an enzyme electrode is described which specifically detects lower primary aliphatic alcohols in aqueous solutions. The electrode consists of a commercial Clark‐type oxygen electrode on which alcohol oxidase (E.C. 1.1.3.13) and catalase were immobilized. The decrease in electrode current is linearly proportional to ethanol concentrations between 1 and 25 ppm. The response of the electrode remains constant during 400 assays over a period of two weeks. The response time is between 1 and 25 min. Assembly of the electrode takes less than 1 h.

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