Premium
The performance of immobilized glucose isomerase supported by shrimp chitin in various types of reactors
Author(s) -
Chen FuSen,
Weng HungShan,
Lai ChingLiang
Publication year - 1983
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260250309
Subject(s) - glucose 6 phosphate isomerase , immobilized enzyme , chitin , chemistry , fluidized bed , thermal diffusivity , chromatography , shrimp , bioreactor , adsorption , isomerization , chemical engineering , michaelis–menten kinetics , continuous stirred tank reactor , isomerase , residence time (fluid dynamics) , enzyme , catalysis , thermodynamics , enzyme assay , biochemistry , organic chemistry , chitosan , biology , geology , physics , geotechnical engineering , fishery , engineering
Five different types of reactors were employed for glucose isomerization using shrimp shell as the support on which to immobilize the glucose isomerase. The Michaelis–Menten constants and effective diffusivity of glucose in the immobilized enzyme bed were experimentally determined and used in a theoretical analysis of the radial‐flow reactor. The fractional conversions of the radial‐flow, fluidizedbed, and packed‐bed reactors with the same ‐residence time were found experimentally to be almost the same. This result reveals that the use of radial‐flow and fluidized‐bed reactors for this immobilized enzyme system is highly feasible.