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Preparation and performance of immobilized yeast cells in columns containing no inert carrier
Author(s) -
Hsiao HumgYu,
Chiang LinChang,
Yang CheMing,
Chen LiFu,
Tsao George T.
Publication year - 1983
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260250206
Subject(s) - yeast , flocculation , chromatography , ethanol , chemistry , yeast extract , population , schizosaccharomyces pombe , yield (engineering) , biomass (ecology) , food science , biochemistry , saccharomyces cerevisiae , biology , fermentation , materials science , organic chemistry , demography , sociology , agronomy , metallurgy
Schizosaccharomyces pombe was cultivated in a medium of glucose (10 g/L) malt extract (3 g/L), yeast extract (3 g/L), and bactopeptone (5 g/L) to form flocs. More than 95% of the cell population were flocculated. Variation in glucose concentration (from 10 to 100 g/L) did not affect flocculation. Yeast extract helped induce flocculation. Application of the immobilized yeast for the continuous production of ethanol was tested in a column reactor. Soft yeast flocs (50–200 mesh) underwent morphological changes to heavy particles (0.1–0.3 cm diameter) after continuously being fed with fresh substrates in the column. Productivity as high as 87 g EtOH L −1 h −1 was obtained when a 150 g/L glucose medium was fed. The performance of this yeast reactor was stable over a two‐month period. The ethanol yield was 97% of the theoretical maximum based upon glucose consumed.