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An enzyme electrode for specific determination of L ‐lysine: A real‐time control sensor
Author(s) -
Tran N. D.,
Romette J. L.,
Thomas D.
Publication year - 1983
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260250203
Subject(s) - lysine , lysine decarboxylase , glutaraldehyde , electrode , gelatin , chemistry , enzyme , chromatography , carbon dioxide , amino acid , biochemistry , organic chemistry , putrescine , cadaverine
Lysine decarboxylase ( L ‐lysine carboxylyase, E.C.4.1.1.18) is immobolized on a carbon dioxide gas‐sensing electrode, by copolymerization with gelatin using the bifuncitional agent glutaraldehyde. The enzyme electrodes thus prepared are used in a continuous flow system to measure the concentration of L ‐lysine in a mixture of amino acids. The measuring time for each sample is about 3 min, including response and rinsing times. The electrode response is linear between 0.01‐1 g/L and has a high specificity for L ‐lysine. The enzyme electrode response to lysine at concentrations below 0.5 g/L is stable on repeated use for at least 500 assays.