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Immobilization of fumarase by entrapment of rat liver mitochondria in polyacrylamide gel using gamma rays
Author(s) -
Godbole S. S.,
Kaul Rajni,
D'Souza S. F.,
Nadkarni G. B.
Publication year - 1983
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260250117
Subject(s) - fumarase , chemistry , malonate , acrylamide , succinic acid , malic acid , biochemistry , succinic anhydride , mitochondrion , sodium , monomer , polyacrylamide gel electrophoresis , enzyme , polyacrylamide , succinates , chromatography , nuclear chemistry , organic chemistry , polymer chemistry , polymer , citric acid
A stable immobilized preparation of fumarase (EC 4.2.1.2) was obtained by entrapment of rat liver mitochondria in acrylamide polymerized by using gamma irradiation (100 kR). The enhanced stability and the efficiency of the entrapped enzyme have shown potential for repeated use for the production of L ‐malic acid from fumaric acid. The possible formation of succinic acid in the system could be controlled by incorporating malonate along with detergents such as sodium deoxycholate or sodium dodecylsulfate in the reactor system.