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Effects of mass‐transfer resistance on apparent stability and performance of fixed‐bed immobilized enzyme reactors: Theory and experiments with immobilized invertase
Author(s) -
Ooshima Hiroshi,
Harano Yoshio
Publication year - 1983
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260250112
Subject(s) - mass transfer , chemistry , diffusion , immobilized enzyme , invertase , chromatography , volumetric flow rate , bioreactor , hydrolysis , continuous stirred tank reactor , sucrose , chemical engineering , thermodynamics , enzyme , organic chemistry , physics , engineering
Taking the hydrolysis of sucrose by invertase immobilized on anion‐exchange resin as an example, the effects of mass‐transfer resistance on the apparent stability of immobilized enzyme (IME) and the optimal policy for an IME reaction in a fixed‐bed reactor have been studied theoretically and experimentally. The following results were obtained: (1) The effect of mass‐transfer resistance on the effective deactivation rate of IME is summarized in two parameters concerning the intraparticle diffusion α p and the interparticle α f . (2) At a constant processed amount of raw materials, there exists an optimal flow rate of reaction fluid to enhance the reactor performance while the mass‐transfer resistance shifts the optimal point. (3) The intrinsic deactivation rate of IME has been estimated from the relationship between the fractional conversion at the reactor outlet and the operation time.