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The effect of the oxygen transfer rate (OSR) on the formation of cellulases by Trichoderma viride in submersion culture
Author(s) -
Škachová Helena,
Gottvaldová Maria,
Kučera Jiří,
Podrazký Vladimíir
Publication year - 1982
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260241207
Subject(s) - cellulose , trichoderma viride , microcrystalline cellulose , fermentation , cellulosic ethanol , cellulase , chemistry , laboratory flask , oxygen , chemical engineering , viscosity , industrial fermentation , materials science , food science , biochemistry , organic chemistry , composite material , engineering
The formation of cellulases by Trichoderma viride in a medium containing cellulose as a sole source of carbon depends on the oxygen transfer rate (OSR); the OSR, on the other hand, depends on the concentration of cellulose in the medium because the concentration of cellulose strongly affects the viscosity of the medium. In the work presented here, the dependence has been determined for the oxygen transfer rate on geometric relations and viscosity in cellulose‐containing media during cultivation in shaken flasks, and the oxygen transfer rate on N Re' N G' and N a during cultivation in a laboratory fermentor of 3000‐mL volume. Two cellulosic materials have been compared with a different effect on viscosity: Microcrystalline beach cellulose and fibrous cellulose. It has been found that, in an applicable range of concentration, microcrystalline cellulose does not affect the oxygen transfer rate (at concentrations up to 3%). Fibrous cellulose increases the OSR during cultivation in shake flasks but decreases it during civilization in fermentors. On the basis of these results, the optimization has been carried out on the cultivation conditions in fermentors.