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Growth of Trichosporon cutaneum under oxygen limitation: Kinetics of oxygen uptake
Author(s) -
Käppeli O.,
Fiechter A.
Publication year - 1982
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260241115
Subject(s) - kinetics , oxygen , chemistry , organic chemistry , physics , quantum mechanics
The relationship between oxygen concentration and growth rate in the yeast Trichosporon cutaneum was studied. In order to establish the conditions for purely oxygen‐limited growth, the cells were first grown in a carbon‐limited chemostat, and kinetic parameters determined. The cells were then grown in an oxygen‐limited chemostat at different dilution rates yielding different oxygen uptake rates. The steady‐state dissolved oxygen tension was found at each dilution rate and the corresponding equilibrium dissolved oxygen tension was found at each dilution rate and the corresponding equilibrium dissolved oxygen concentration determined in the effluent medium. The relationship between oxygen concentration and growth rate followed Monod‐type kinetics with an apparent K O of 4.38 × 10 −6 M .