Premium
The kinetic of lactose hydrolysis for the β‐galactosidase from Aspergillus niger
Author(s) -
Flaschel E.,
Raetz E.,
Renken A.
Publication year - 1982
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260241114
Subject(s) - aspergillus niger , mutarotation , lactose , lactase , galactose , chemistry , hydrolysis , anomer , kinetics , beta galactosidase , non competitive inhibition , stereochemistry , enzyme , chromatography , biochemistry , physics , gene expression , quantum mechanics , gene
The kinetics of glucose liberation from lactose by means of the β‐glactosidase from Aspergillus niger has been studied in a wide range of the main variables. The analysis shows that the kinetic models proposed so far are not adequate. The main finding is that the reaction rate is not linearly correlated to the enzyme concentration—it increase more than proportionally. This nonlinear relationship results because this lactase can distinguish between α‐and β‐galactose α‐Galactose acts as competitive and anticompetitive inhibitor while β‐galactose is a competitive one. The competitive inhibition of the α‐anomer is approximately 12 times more sever than that of the β‐anomer. The kinetics, including a simplified model for the mutarotation of galactose is given for a temperature of 50°C at a pH of 3.5—the most likely conditions for the application of this lactase in acid whey treatment.