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Cell immobilization in k ‐carrageenan with tricalcium phosphate
Author(s) -
Wang Henry Y.,
Hettwer David J.
Publication year - 1982
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260240809
Subject(s) - carrageenan , phosphate , yeast , chemistry , ethanol , chromatography , matrix (chemical analysis) , calcium , biophysics , chemical engineering , nuclear chemistry , biochemistry , biology , organic chemistry , engineering
The objectives of this research were to investigate the growth of immobilized yeast cells in k ‐carrageenan gel and study the effect of trapping hydroxyapatite (tricalcium phosphate) crystals into the matrix. Using k ‐carrageenan, the final number of cells per milliliter of gel is at least an order of magnitude higher than free cells per milliliter of medium. A “cell retention” theory explaining this cell concentration difference was proposed. Coexistence of yeast cells and an additional agent such as tricalcium phosphate results in sustained viability through internal pH control, increased cell loading, greater settling velocity, and enhanced ethanol production.