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Use of chemically modified activated carbon as a support for immobilized enzymes
Author(s) -
Osborn James A.,
Ianniello Robert M.,
Wieck Henry J.,
Decker Thomas F.,
Gordan Susan L.,
Yacynych Alexender M.
Publication year - 1982
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260240716
Subject(s) - carbodiimide , cyanuric chloride , covalent bond , chemistry , glucose oxidase , immobilized enzyme , activated carbon , enzyme , carbon fibers , chymotrypsin , biochemistry , organic chemistry , trypsin , adsorption , materials science , composite number , composite material
Loading and activity assays of the enzymes α‐chymotrypsin, α‐chymotrypsinogen, and glucose oxidase covalently bound to an activated carbon support are presented. The activated carbon support material was pretreated using either a radio‐frequency oxygen plasma or an electrochemical oxidation to maximize the enzyme attachment. Cyanuric chloride or water‐soluble carbodiimide linking reactions were used to covalently attach the enzymes to the carbon support. Discussion of the relative merits of each reaction scheme is presented.